The genus Holonuncia Forster and the type species H. cavernicola Forster are redescribed. One new combination is established: H. seriata (Nuncia) (Roewer, 1915). Holonuncia seriata and H. tuberculata (Roewer, 1915) are redescribed and ten new species described: H. sussa, H. dewae, H. francesae, H. katoomba, H. weejasperensis, H. dispar, H. recta, H. hamiltonsmithi, H. kaputarensis and H. enigma. Allozyme electrophoresis resolves some species boundary problems. Morphological adaptations to cave life include leg attenuation, depigmentation and eye regression but not loss. The distribution of the genus in eastern mainland Australian caves is illustrated.