Toxotes kimberleyensis n.sp. is described from 22 specimens, 52.5-126.3 mm SL, collected from freshwater streams in the Kimberley region of northwestern Australia. It was previously identified as Toxotes oligolepis Bleeker, a poorly known species from Indonesia. However, re-examination of Bleeker's type specimen indicates significant differences between the two species relating to the length of the dorsal spines, and lateral-line scale count. A key to the seven species of Toxotes is provided.